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mcp 1 ek287  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd mcp 1 ek287
    Mcp 1 Ek287, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mcp 1 ek287/product/Multi Sciences (Lianke) Biotech Co Ltd
    Average 94 stars, based on 33 article reviews
    mcp 1 ek287 - by Bioz Stars, 2026-02
    94/100 stars

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    Image Search Results


    AAVs induce dose-dependent CSF dyshomeostasis. (A) CSF CCL2 concentrations measured by ELISA at P7.5 following P0.5 ICV injection of escalating AAV doses. (B, C) Parenchymal microglial response. (B) Representative Iba1 immunostaining in P7.5 brain sections. (C) Quantification of Iba1⁺ cell density in parenchymal regions. (D, E) ChP microglial response. (D) Representative Iba1 immunostaining in the ChP. (E) Quantification of Iba1⁺ cell density in the ChP. (F) CSF transthyretin (TTR) concentrations measured by ELISA at P7.5 following P0.5 ICV injection of escalating AAV doses. (G–J) ChP protein analysis by immunoblot. (G) Representative blot for TTR and GFP in ChP lysates. (H) Relative GFP expression. (I) Relative TTR expression. (J) Correlation analysis between GFP and TTR levels in individual samples. (K, L) CSF ion disruption following inflammation. (K) Experimental timeline for LPS-induced inflammation and CSF collection in adult mice. (L) CSF potassium (K⁺) concentration measured by ICP-MS.

    Journal: bioRxiv

    Article Title: Adeno-associated viruses (AAVs) induce dose-dependent neonatal ventriculomegaly following intracerebroventricular administration

    doi: 10.64898/2025.12.30.697113

    Figure Lengend Snippet: AAVs induce dose-dependent CSF dyshomeostasis. (A) CSF CCL2 concentrations measured by ELISA at P7.5 following P0.5 ICV injection of escalating AAV doses. (B, C) Parenchymal microglial response. (B) Representative Iba1 immunostaining in P7.5 brain sections. (C) Quantification of Iba1⁺ cell density in parenchymal regions. (D, E) ChP microglial response. (D) Representative Iba1 immunostaining in the ChP. (E) Quantification of Iba1⁺ cell density in the ChP. (F) CSF transthyretin (TTR) concentrations measured by ELISA at P7.5 following P0.5 ICV injection of escalating AAV doses. (G–J) ChP protein analysis by immunoblot. (G) Representative blot for TTR and GFP in ChP lysates. (H) Relative GFP expression. (I) Relative TTR expression. (J) Correlation analysis between GFP and TTR levels in individual samples. (K, L) CSF ion disruption following inflammation. (K) Experimental timeline for LPS-induced inflammation and CSF collection in adult mice. (L) CSF potassium (K⁺) concentration measured by ICP-MS.

    Article Snippet: The concentrations of C-C motif chemokine ligand 2 (CCL2) and transthyretin (TTR) in the CSF were quantified using commercial ELISA kits (mouse CCL2 ELISA kit, BSKM1011, Bioss; mouse TTR ELISA kit, ab282297, Abcam), according to the manufacturers’ protocols.

    Techniques: Enzyme-linked Immunosorbent Assay, Injection, Immunostaining, Western Blot, Expressing, Disruption, Concentration Assay

    AAV induces dose-dependent embryonic and adult ventriculomegaly. (A) Experimental timeline for assessing acute AAV2/5 transfection 24 hours post in utero ICV injection. (B) Representative image confirming robust AAV2/5 transfection in the embryonic ChP at E14.5 (24 hours post-injection). (C) Timeline for evaluating the effects of escalating AAV2/5 doses via in utero ICV injection at E13.5, with analysis at E16.5. (D) Embryonic survival rates at E16.5. Numbers indicate the proportion of surviving embryos per litter. (E) Representative coronal sections of E16.5 embryonic brains showing transgene expression (GFP) and ventricular morphology. (F, G) CSF analysis from E16.5 embryos. (F) CCL2 concentrations. (G) Transthyretin (TTR) concentrations. (H) Timeline for assessing the effects of escalating AAV2/5 doses via ICV injection in adult mice (3-day endpoint). (I) Representative coronal sections at the level of the posterior anterior commissure (pAC; dashed outline) showing lateral ventricle morphology in adult brains. (J, K) Representative sagittal section demonstrating the differential cellular tropism of AAV2/5. (J) ChP-specific transfection by low-dose AAV2/5. (K) A more permissive tropism of high-dose AAV2/5, with ectopic GFP signal also observed in hippocampal CA1 region.

    Journal: bioRxiv

    Article Title: Adeno-associated viruses (AAVs) induce dose-dependent neonatal ventriculomegaly following intracerebroventricular administration

    doi: 10.64898/2025.12.30.697113

    Figure Lengend Snippet: AAV induces dose-dependent embryonic and adult ventriculomegaly. (A) Experimental timeline for assessing acute AAV2/5 transfection 24 hours post in utero ICV injection. (B) Representative image confirming robust AAV2/5 transfection in the embryonic ChP at E14.5 (24 hours post-injection). (C) Timeline for evaluating the effects of escalating AAV2/5 doses via in utero ICV injection at E13.5, with analysis at E16.5. (D) Embryonic survival rates at E16.5. Numbers indicate the proportion of surviving embryos per litter. (E) Representative coronal sections of E16.5 embryonic brains showing transgene expression (GFP) and ventricular morphology. (F, G) CSF analysis from E16.5 embryos. (F) CCL2 concentrations. (G) Transthyretin (TTR) concentrations. (H) Timeline for assessing the effects of escalating AAV2/5 doses via ICV injection in adult mice (3-day endpoint). (I) Representative coronal sections at the level of the posterior anterior commissure (pAC; dashed outline) showing lateral ventricle morphology in adult brains. (J, K) Representative sagittal section demonstrating the differential cellular tropism of AAV2/5. (J) ChP-specific transfection by low-dose AAV2/5. (K) A more permissive tropism of high-dose AAV2/5, with ectopic GFP signal also observed in hippocampal CA1 region.

    Article Snippet: The concentrations of C-C motif chemokine ligand 2 (CCL2) and transthyretin (TTR) in the CSF were quantified using commercial ELISA kits (mouse CCL2 ELISA kit, BSKM1011, Bioss; mouse TTR ELISA kit, ab282297, Abcam), according to the manufacturers’ protocols.

    Techniques: Transfection, In Utero, Injection, Expressing